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Journal / Immunology and Infection / Isolatie van tonsillaire mononucleaire cellen om…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolatie van tonsillaire mononucleaire cellen om ex vivo ingeboren immuunreacties te bestuderen in een menselijk mucosale lymfoïde weefsel
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Isolatie van tonsillaire mononucleaire cellen om ex vivo ingeboren immuunreacties te bestuderen in een menselijk mucosale lymfoïde weefsel

Article DOI: 10.3791/60914-v • 07:38 min • June 14th, 2020
June 14th, 2020 •
1,2,3,4, 2,3,4, 4,5, *6, *2,3,4
1Institute of Molecular Virology, Ulm University Medical Center, 2CNRS UMR-8601, Centre Interdisciplinaire Chimie Biologie, 3Team Chemistry & Biology, Modeling & Immunology for Therapy, Université Paris Descartes, 4Université Paris Descartes, Sorbonne Paris Cité, 5Pediatric Otolaryngology Department, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Université de Paris, Institut Cochin
* These authors contributed equally

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In het huidige protocol leggen we uit hoe je gemakkelijk tonsillar mononucleaire cellen van gezonde mensen verwerken en kweken die gedeeltelijke chirurgische tonsillectomie ondergaan om aangeboren immuunreacties bij activering te bestuderen, waarbij virale infectie in mucosale weefsels nabootst.

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Immunologie en infectie probleem 160 slijmvlies-geassocieerde lymfoïde weefsel amandel tonsillar mononucleaire cel immuniteit ex vivo celcultuur cytokine gastheer-pathogene interactie
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