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Generazione di un chip di barriera emato-encefalica basata sull'iPSC umano
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1The Department of Physiology and Cell Biology, Faculty of Health Sciences,Ben-Gurion University of the Negev, 2The Regenerative Medicine and Stem Cell (RMSC) Research Center,Ben-Gurion University of the Negev, 3The Zlotowski Center for Neuroscience,Ben-Gurion University of the Negev, 4The Board of Governors Regenerative Medicine Institute,Cedars-Sinai Medical Center, 5Division of Micro and Nanosystems,KTH Royal Institute of Technology, 6AIMES, Department of Neuroscience,Karolinska Institutet
Chapters
- 00:05Introduction
- 01:27Seeding the “Brain Side” Channel and Differentiating EZ Spheres into Mixed Neural Cultures
- 03:04Seeding iBMECs (iPSC-Derived Brain Microvascular Endothelial Cells) into the Bottom Channel to Generate the “Blood Side”
- 05:27Initiation of Flow
- 08:12Results: Immunocytochemistry and Permeability of the iPSC-Base Blood-Brain Barrier Chip
- 09:17Conclusion
La barriera emato-encefalica (BBB) è un'unità neurovascolare multicellulare che regola strettamente l'omeostasi cerebrale. Combinando gli iPSC umani e le tecnologie organ-on-chip, abbiamo generato un chip BBB personalizzato, adatto per la modellazione delle malattie e le previsioni di penetrazione dei farmaci del SNC. Viene descritto un protocollo dettagliato per la generazione e il funzionamento del chip BBB.
