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Multiphoton Microscopic Observation of Vessels in Mouse Liver Tissue
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Multiphoton Microscopic Observation of Vessels in Mouse Liver Tissue
DOI:

06:33 min

May 17, 2021

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Chapters

  • 00:05Introduction
  • 00:39Mouse Preparation
  • 01:39Liver Imaging Framing
  • 02:35Multiphoton Laser Scanning Microscope Setup
  • 03:53Multiphoton Laser Scanning Micrography
  • 05:07Results: Representative Body Organ Frame Imaging
  • 05:54Conclusion

Summary

Automatic Translation

In this experiment, a mouse is injected in its tail vein with Rhodamine B isothiocyanate–dextran that can stain blood vessels. After the liver is exposed and fixed, a specific part of the liver can be selected to observe the deep tissue in the living body using multiphoton microscopy.

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