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Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem
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Developmental Biology
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JoVE Journal Developmental Biology
Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem

Live Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem

DOI:
10.3791/60936-v

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07:52 min

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May 23, 2020

•

,
1Max Planck Institute of Molecular Plant Physiology

Chapters

  • 00:04Introduction
  • 00:34Plant Growth
  • 01:18Shoot Apical Meristem (SAM) Dissection
  • 02:14Cultured SAM Transfer and Growth
  • 02:57SAM Imaging
  • 04:57Micromechanical SAM Perturbation and Data Analysis
  • 06:23Results: Representative Cortical Microtubule Anisotropy Quantification and Mechanical Ablation in Arabidopsis SAM
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a protocol for live cell imaging of the cortical microtubule cytoskeleton at the shoot apical meristem and monitoring its response to changes in physical forces.

Tags

Keywords: Microtubule CytoskeletonLive Cell ImagingArabidopsisShoot Apical MeristemMechanical ManipulationPlant GrowthPlant DevelopmentDissectionMicroscopyPlant Genetics

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