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Journal / Biology / Isolation of Cardiomyocytes from Fixed Hearts for …
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Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis

Article DOI: 10.3791/60938-v • 08:41 min • October 7th, 2020
October 7th, 2020 •
*1,2, *2, 1,2,3
1Department of Integrative Biology and Physiology, University of Minnesota, 2Lillehei Heart Institute, Department of Medicine, University of Minnesota, 3Stem Cell Institute, University of Minnesota
* These authors contributed equally

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Summary
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The goal of this work is to develop a method to reproducibly isolate cardiomyocytes from the adult heart and measure DNA content and nucleation.

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Cardiomyocytes Fixed Hearts Isolation Immunocytochemistry Ploidy Analysis Protocol Cell Yields Cell Morphology Nucleation Status Visual Verification Euthanized Mouse Supine Position Chest Incision Descending Aorta Inferial Vena Cava Peristaltic Pump Potassium Chloride PBS Solution 4%PFA Solution Centrifuge Tube Incubation Petri Dish
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