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JoVE Journal
Bioengineering

This content is Open Access.

Traction Force Microscopy to Study B Lymphocyte Activation
 

Traction Force Microscopy to Study B Lymphocyte Activation

Article DOI: 10.3791/60947-v
July 23rd, 2020

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Summary July 23rd, 2020

Here we present a protocol used to perform traction force microscopy experiments on B cells. We describe the preparation of soft polyacrylamide gels and their functionalization, as well as data acquisition at the microscope and a summary of data analysis.

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