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Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes
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Immunology and Infection
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JoVE Journal Immunology and Infection
Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes

Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes

DOI:
10.3791/60949-v

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08:52 min

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February 05, 2021

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, , ,
1Department of Pathology, Renaissance School of Medicine,Stony Brook University, Stony Brook, NY, 2Stony Brook Cancer Center, Stony Brook, NY, 3Department of Medicine,Weill Cornell Medicine, New York, NY

Chapters

  • 00:05Introduction
  • 00:46Colonoscopy and Wound Induction
  • 02:46Wound Bed Visualization and Measurement
  • 04:37Wound Bed Collection
  • 06:02Histological Analysis Preparation
  • 07:45Results: Representative Wound Bed Visualization
  • 08:21Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we provide a detailed description of the procedure to induce colonoscopic-guided pinch biopsies in mice and track wound closure in real time. Additionally, methods for the preparation of tissues for histological, immunohistochemical and molecular analyses of the wound bed are provided.

Tags

Keywords: Colonoscopic-guided Pinch BiopsyMiceColonic Wound HealingInflammatory Bowel Disease (IBD) PathogenesisEndoscopeBiopsy ForcepsAnesthesiaPBSVideo Recording

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