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Cancer Research

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JoVE Journal Cancer Research
Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion
Counting Proteins in Single Cells with Addressable Droplet Microarrays
Journal (Cancer Research)
Counting Proteins in Single Cells with Addressable Droplet Microarrays
Droplet Digital TRAP (ddTRAP): Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction
Journal (Cancer Research)
Droplet Digital TRAP (ddTRAP): Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction
 

Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion

Article DOI: 10.3791/60997-v • 09:23 min • April 2nd, 2020
April 2nd, 2020 •
1, 1, 1,2
1Children's Mercy Research Institute, Children's Mercy Kansas City, 2Department of Pediatrics, University of Missouri Kansas City School of Medicine

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Summary
Automatic Translation

Cancer is a lethal disease due to its ability to metastasize to different organs. Determining the ability of cancer cells to migrate and invade under various treatment conditions is crucial to assessing therapeutic strategies. This protocol presents a method to assess the real-time metastatic abilities of a glioblastoma cancer cell line. 

Tags

Impedance-based Measurement Real-time Cancer Cell Migration Invasion Gene Function Drugs Cell Kinetics Staining Mechanical Cell Damage Fluorescent Markers U-118MG Cell Line Culture PBS Trypsin-EDTA Centrifugation Resuspension Buffer R SiRNA Electroporation Culture Medium
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