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Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging
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Biochemistry
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JoVE Journal Biochemistry
Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

DOI:
10.3791/61004-v

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09:31 min

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March 27, 2020

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, , , ,
1Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund Berlin, 2NeuroCure Cluster of Excellence,Charité – Universitätsmedizin Berlin, 3Molecular Neuroscience Group, Department of Chemical Engineering and Biotechnology,University of Cambridge, 4Cell Biology,University of Bremen

Chapters

  • 00:05Introduction
  • 01:25Synchronization of C. elegans and Mounting Nematodes onto Microscopy Slides
  • 02:58Acquisition of FLIM (Fluorescence Lifetime Imaging Microscopy) Data
  • 04:32Analysis of FLIM Data Using FLIMfit Software
  • 07:15Results: Fluorescence Lifetimes
  • 08:15Conclusion

Summary

Automatic Translation

Automatic Translation

Fluorescence lifetime imaging monitors, quantifies and distinguishes the aggregation tendencies of proteins in living, aging, and stressed C. elegans disease models.

Tags

Keywords: Amyloid StructuresFluorescence Lifetime ImagingProtein AggregationC. ElegansNeurodegenerative DiseasesFLIMAgarose PadsNematodes

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