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JoVE Journal Biochemistry
Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins
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Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins

Article DOI: 10.3791/61018-v • 08:28 min • March 29th, 2020
March 29th, 2020 •
1, 1
1Department of Pharmacology, UC Davis School of Medicine

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Palmitoylation entails the incorporation of a 16-carbon palmitate moiety to cysteine residues of target proteins in a reversible manner. Here, we describe a biochemical approach, the acyl-PEGyl exchange gel shift (APEGS) assay, to investigate the palmitoylation state of any protein of interest in mouse brain lysates.

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Acyl-PEGyl Exchange Gel Shift Assay Quantitative Determination Palmitoylation Brain Membrane Proteins Biochemical Method Affinity Purification Gel Mobility Modifications Homogenization Buffer Glass Homogenizer Centrifuge Lysates Supernatant Pellet Suspension Membrane Fraction Pipette Tip ABCA Assay APEGS Assay
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