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Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus
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Immunology and Infection
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JoVE Journal Immunology and Infection
Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus

Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus

DOI:
10.3791/61025-v

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07:47 min

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May 18, 2020

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, , , ,
1Department of Biotechnology, Faculty of Applied Science,King Mongkut’s University of Technology North Bangkok (KMUTNB), 2Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine,Kasetsart University, 3Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies,Kasetsart University, 4Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS),King Mongkut’s University of Technology North Bangkok (KMUTNB)

Chapters

  • 00:03Introduction
  • 00:59RNA Extraction
  • 02:50Primer Design
  • 05:20Demonstration of RT-Lamp Assay
  • 07:10Results
  • 08:19Conclusion

Summary

Automatic Translation

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We present an RT-LAMP assay for the detection of TiLV in tilapia fish using simple instruments over a relatively short period of time compared to conventional RT-PCR techniques. This protocol may help control the epidemic spread of TiLVD, especially in developing countries.

Tags

Keywords: RT-LAMPReverse Transcription Loop-mediated Isothermal AmplificationTilapia Lake VirusTiLVRapid DetectionRNA ExtractionPrimer DesignPrimerExplorerLAMP PrimersSegment 3AquacultureTilapiaVirus Detection

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