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Journal / Immunology and Infection / Reverse Transcription Loop-Mediated Isothermal…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus
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Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus

Article DOI: 10.3791/61025-v • 07:47 min • May 18th, 2020
May 18th, 2020 •
1, 2,3, 2, 4, 2,3
1Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok (KMUTNB), 2Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, 3Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, 4Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS), King Mongkut's University of Technology North Bangkok (KMUTNB)

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Summary
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We present an RT-LAMP assay for the detection of TiLV in tilapia fish using simple instruments over a relatively short period of time compared to conventional RT-PCR techniques. This protocol may help control the epidemic spread of TiLVD, especially in developing countries.

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Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay Tilapia Lake Virus Emerging Virus Contagious Pathogens High Mortality Tilapia Aquaculture Food Security Fast Detection Method Accurate Detection Method Sensitive Detection Method Fish Tissue Disease Control Impact Reduction RNA Extraction Liver Tissue Acid Guanidinium-phenol Base Reagent Mince Centrifuge Supernatant 95% Ethanol Spin Column Collection Tube
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