Measuring Real-time Drug Response in Organotypic Tumor Tissue Slices

Our protocol is compatible with other molecular analysis methods and allows the assessment of how drugs affect the viability of in-tact tissue slices and the performance of medium throughput screening. The main advantage of this technique is that we can measure the viability of a whole tissue slice in real time with very little pre-or post-processing. This technique accelerate pre-clinical drug development and oncology by testing drug candidates or tissues slices acquired directly from patient tumor tissue samples.

Our real-time tissue viability assessment method is quite versatile and can be applied to cancer biology, neuroscience, and developmental biology studies. Preparing in-tact viable tissue slices is the key to the success of the procedure. The Vibratome settings in medium composition should be adjusted according to the tissue type and firmness.