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In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding
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Bioengineering
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JoVE Journal Bioengineering
In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

DOI:
10.3791/61037-v

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08:20 min

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May 01, 2020

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, , , , , , ,
1Skin Research Institute of Singapore,Agency for Science, Technology and Research (A*STAR), 2School of Chemical and Life Sciences,Singapore Polytechnic, 3Institute of Medical Biology,Agency for Science, Technology and Research (A*STAR)

Chapters

  • 00:04Introduction
  • 00:50Construction of MMC Hypertrophic Scar Model
  • 01:47Expression of Total Collagen
  • 02:47Expression of Collagen I
  • 03:47Western Blotting
  • 05:04RT-PCR
  • 05:43Results: Effects of MMC on Cell Protein and Gene Expression
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the use of macromolecular crowding to create an in vitro human hypertrophic scar tissue model that resembles in vivo conditions. When cultivated in a crowded macromolecular environment, human skin fibroblasts exhibit phenotypes, biochemistry, physiology, and functional characteristics resembling scar tissue.

Tags

Keywords: In Vitro ModelHypertrophic ScarringMacromolecular CrowdingCollagen-rich EnvironmentFibroblastsSirius Red StainingExtracellular MatrixPulmonary Fibrosis

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