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Immunology and Infection

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JoVE Journal Immunology and Infection
Forenklet Reverse Genetics metode til at inddrive rekombinant rotavira udtrykke Reporter Proteiner
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Forenklet Reverse Genetics metode til at inddrive rekombinant rotavira udtrykke Reporter Proteiner

Article DOI: 10.3791/61039-v • 11:40 min • April 17th, 2020
April 17th, 2020 •
1, 1, 1, 1
1Department of Biology, Indiana University

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Generation af rekombinant rotavira fra plasmid DNA er et vigtigt redskab til undersøgelse af rotavirus replikation og patogenese, og udviklingen af rotavirus udtryk vektorer og vacciner. Heri beskriver vi en forenklet omvendt genetik tilgang til at generere rekombinant rotavira, herunder stammer, der udtrykker fluorescerende reporter proteiner.

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Immunologi og infektion Problem 158 rotavirus omvendt genetik fluorescerende protein rekombinant virus rekombinant rotavira viral ekspressionsvektor
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