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JoVE Journal
Immunology and Infection

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Forenklet Reverse Genetics metode til at inddrive rekombinant rotavira udtrykke Reporter Proteiner
 
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Forenklet Reverse Genetics metode til at inddrive rekombinant rotavira udtrykke Reporter Proteiner

Article DOI: 10.3791/61039-v
April 17th, 2020

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Summary April 17th, 2020

Please note that all translations are automatically generated.

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Generation af rekombinant rotavira fra plasmid DNA er et vigtigt redskab til undersøgelse af rotavirus replikation og patogenese, og udviklingen af rotavirus udtryk vektorer og vacciner. Heri beskriver vi en forenklet omvendt genetik tilgang til at generere rekombinant rotavira, herunder stammer, der udtrykker fluorescerende reporter proteiner.

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