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Application of Atomic Force Microscopy to Detect Early Osteoarthritis
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Bioengineering
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JoVE Journal Bioengineering
Application of Atomic Force Microscopy to Detect Early Osteoarthritis

Application of Atomic Force Microscopy to Detect Early Osteoarthritis

DOI:
10.3791/61041-v

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09:22 min

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May 24, 2020

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, , ,
1Laboratory of Cell Biology, Department of Orthopaedic Surgery,University Hospital of Tübingen, 2Medical faculty of the University of Tübingen, 3Department of Orthopaedic Surgery,University Hospital of Tübingen

Chapters

  • 00:04Introduction
  • 00:47Cartilage Cryotome Sectioning
  • 01:36Cartilage Sample Atomic Force Microscopy (AFM) Preparation
  • 02:17AFM Device Preparation
  • 03:14Cantilever Sample and Calibration
  • 05:25Biomechanical Extracellular Matrix (ECM) and Pericellular Matrix (PCM) Characterization
  • 07:04Data Processing
  • 07:46Results: Representative Relationships Between Young s Moduli and Cellular Spatial Organization
  • 08:29Conclusion

Summary

Automatic Translation

Automatic Translation

We present a method to investigate early osteoarthritic changes at the cellular level in articular cartilage by using atomic force microscopy (AFM).

Tags

Keywords: Atomic Force MicroscopyOsteoarthritisBiomechanical PropertiesElasticityArticular CartilageCryotomePBSSample GlueLeibovitz’s L-15 MediumAFM CantileverMicrosphere Probe

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