Journal
/
Immunology and Infection
/
Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

DOI:
10.3791/61049-v

•

09:25 min

•

May 24, 2020

•

, , , ,
1Department of Medial Microbiology and Immunology,School of Medicine, University of California Davis, 2Department of Microbiology and Immunology,University of Texas Medical Branch at Galveston

Chapters

  • 00:04Introduction
  • 01:03Recombination Vector Generation
  • 03:47Recombinant Virus Generation
  • 06:31Results: Representative Recombinant Poxvirus Generation
  • 08:36Conclusion

Summary

Automatic Translation

Automatic Translation

This is a method to generate “scarless” recombinant vaccinia viruses using host-range selection and visual identification of recombinant viruses.

Tags

Recombinant PoxvirusesHost Range SelectionFluorescent MarkersHomologous RecombinationTransient Dominant SelectionVaccinia VirusForeign Gene ExpressionVaccinesPCRDNA Gel ExtractionDNA AssemblyE. Coli Transformation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Generation of Recombinant Influenza Virus from Plasmid DNA

RNAi Screening for Host Factors Involved in Vaccinia Virus Infection using Drosophila Cells

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

Generation of Recombinant Arenavirus for Vaccine Development in FDA-Approved Vero Cells

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions

Visual and Microscopic Evaluation of Streptomyces Developmental Mutants

Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

Read Article