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Optimalisatie voor sequencing en analyse van gedegradeerde FFPE-RNA Samples
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Optimalisatie voor sequencing en analyse van gedegradeerde FFPE-RNA Samples

Article DOI: 10.3791/61060-v • 07:30 min • June 8th, 2020
June 8th, 2020 •
*1, *1,2, 1, 1, 1,2, 1
1NCI CCR Sequencing Facility, Frederick National Laboratory for Cancer Research, 2Advanced Biomedical and Computational Sciences, Frederick National Laboratory for Cancer Research
* These authors contributed equally

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Deze methode beschrijft de stappen om de kwaliteit en kwantiteit van sequentiegegevens te verbeteren die kunnen worden verkregen uit formeel vaste paraffine-embedded (FFPE) RNA-monsters. We beschrijven de methodologie om de kwaliteit van FFPE-RNA-monsters nauwkeuriger te beoordelen, sequencingbibliotheken voor te bereiden en de gegevens van FFPE-RNA-monsters te analyseren.

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Genetica RNA sequencing formaline-fixed paraffine embedded FFPE next generation sequencing NGS RNA-seq analysis
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