Name: In Situ Chemotaxis Assay to Examine Microbial Behavior in Aquatic Ecosystems (Video) | JoVE Journal
Uploaded: 2020-05-05T14:45:00.000+00:00
Duration: 7 min 23 s
Description: Learn in situ chemotaxis assay methods to study microbial behavior in aquatic ecosystems using the ISCA microfluidic device. This protocol enables robust quantification of chemotaxis and isolation of microorganisms.

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This research was funded in part by the Gordon and Betty Moore Foundation Marine Microbiology Initiative, through grant GBMF3801 to J.R.S. and R.S., and an Investigator Award (GBMF3783) to R.S., as well as an Australian Research Council Fellowship (DE160100636) to J.B.R., an award from the Simons Foundation to B.S.L. (594111), and a grant from the Simons Foundation (542395) to R.S. as part of the Principles of Microbial Ecosystems (PriME) Collaborative.

We recommend executing section 1 prior to field experiments to optimize results.
1. Laboratory optimization
NOTE: The volumes described in the optimization procedure are sufficient for a single ISCA (composed of 20 wells).
<ol>
	<li>Preparation of the chemical of interest 
	NOTE: The optimal concentration for each chemoattractant often needs to be determined under laboratory conditions prior to field deployments. The chemical concentration field will decrease in magnitude with distance from the source (ISCA well), which means that the concentration experienced by microorganisms in the environment will be lower than that present inside the device. The optimal concentration to use in the ISCA well depends on the diffusion rate of the chemoattractant. If the concentration in the well is too low (close to the detection limit of the microbes), no positive chemotaxis will be observed. Conversely, if the concentration in the well is too high, the concentration will also be high in the immediate environment and microbial accumulation will occur above the ISCA wells rather than inside. Therefore, dilution series should be carried out in laboratory conditions for each compound in order to determine the optimal concentration for field deployments. Ideally, a concentration range should also be tested in the field to confirm laboratory results.
	<ol>
		<li>Prepare 2.5 L of a suitable medium containing the appropriate salt concentration (e.g., phosphate-buffered saline [PBS] or artificial seawater). Filter the medium with a 0.2 &#181;m filter using a peristaltic or vacuum pump and autoclave.</li>
		<li>Prepare a 10 mM solution of chemoattractant in 1 mL of the sterile medium. Filter the chemoattractant solution with a 0.2 &#181;m syringe filter to remove particles and potential contaminants. 
		NOTE: Ideally, no organic compounds other than the chemoattractant should be present in the final solution.</li>
	</ol>
	</li>
	<li>Concentration range by dilution series
	<ol>
		<li>Dilute the filtered chemoattractant stock solution in series, ranging (for example) from 10 mM to 100 &#181;M. 
		NOTE: At least a 0.7 mL final volume is needed per concentration tested.</li>
	</ol>
	</li>
	<li>Loading the ISCA
	<ol>
		<li>Fill a 1 mL syringe with the filtered chemoattractant and connect it to a 27 G syringe. Holding the ISCA with the port facing upward, slowly inject the substance until a small droplet appears on top of the port. 
		NOTE: 1) Each dilution or substance must be injected with a separate syringe and needle to prevent cross-contamination. 2) This small droplet is important because it ensures that no air bubble is trapped within the port, which could impair the ability of microbes to migrate through the port. 3) It is recommended to fill an entire row per substance or concentration (five wells) to provide an adequate minimal volume for further analyses. 4) One row per ISCA should act as negative control and should be filled with the filtered medium in which the microbes will be suspended. This treatment accounts for the effect of random motility by microbes into the ISCA wells and should be used to normalize the treatments containing a chemoattractant.</li>
	</ol>
	</li>
	<li>Deployment in the laboratory
	<ol>
		<li>Overnight, incubate a 5 mL culture enriched with 1% marine broth (for marine bacteria) or 1% lysogeny broth (LB, for fresh water bacteria). 
		NOTE: Motile bacterial isolates or natural bacterial communities can be used for laboratory deployments.</li>
		<li>Incubate the culture for 12 h at room temperature (RT) and 180 rpm. After 12 h, ensure that the microbial communities are motile by direct observation under a microscope.</li>
		<li>Spin down the culture at 1,500 x g for 10 min and resuspend 1/100 in 150 mL of appropriate medium (e.g., filtered seawater, filtered freshwater).</li>
		<li>Place two small pieces of double-sided adhesive tape on the flat surface of a 200 mL capacity tray (the lids of 1 mL tip boxes have the ideal dimensions for this purpose and can easily be autoclaved). Place one ISCA on top, ensuring that it attaches securely to the tape. Slowly fill the deployment tray with the bacterial solution using a 50 mL serological pipette. 
		NOTE: Fill the tray until the ISCA is submerged under approximately 1&#8211;2 cm of liquid. If using multiple trays, use the same volume across all.</li>
		<li>Leave the ISCA to incubate for 1 h to allow bacterial chemotaxis. After 1 h, remove the medium very gently with a 50 mL serological pipette to minimize turbulent flow.</li>
		<li>Retrieve the ISCA from the deployment tray without touching the upper surface. Use a pipette and disposable wipers to remove remaining liquid on the ISCA surface. 
		NOTE: It is important to avoid touching the ports during this process, as the resulting changes in pressure can remove or add bacteria from the outside environment into the well and thereby bias the bacterial density and composition inside the well.</li>
	</ol>
	</li>
	<li>Retrieval of the samples
	<ol>
		<li>Holding the ISCA with the port facing downward, retrieve the volume of the wells using a sterile 27 G syringe needle attached to a 1 mL syringe. 
		NOTE: Each row (if containing the same substance) can be pooled to provide a working sample of approximately 550 &#181;L. This sample can subsequently be aliquoted into different tubes depending on the required downstream applications.</li>
		<li>Determine the number of bacteria attracted to each chemoattractant concentration by analyzing the samples with flow cytometry12. Choose the concentration of chemoattractant that maximizes chemotaxis for subsequent field deployments.</li>
	</ol>
	</li>
</ol>
2. Preparation for field deployment
NOTE: Preparation of material and construction of the flow-damping enclosure (section 2) must be conducted prior to deployment.
<ol>
	<li>Preparation of materials
	<ol>
		<li>Prepare all materials listed in Table 1. 
		NOTE: Material quantities are provided for one ISCA.</li>
	</ol>
	</li>
	<li>Construction and preparation of the flow-damping enclosure 
	NOTE: The flow-damping enclosure minimizes unwanted turbulences that otherwise prevents the establishment of chemical gradients emanating from the ISCA.
	<ol>
		<li>Cut the pieces for the deployment enclosure with a laser cutter from a 3 mm acrylic sheet. 
		NOTE: The file for the pieces can be found using the following link: &#60;https://figshare.com/articles/Flow_damping_enclosure_for_ISCA_deployments/10630220&#62;.</li>
		<li>Assemble the laser-cut pieces as demonstrated in Figure 2 using acrylic glue. 
		NOTE: Assemble the pieces with care. Holes or misalignment can create leaks upon deployment, which directly impacts data quality.</li>
		<li>Leave the assembled enclosure to dry overnight.</li>
		<li>Wash the enclosure with deionized water.</li>
		<li>Identify potential leakage by pouring deionized water into the enclosure. Fix any potential leaking joints by adding more acrylic glue, then repeat steps 2.2.3&#8211;2.2.5.</li>
		<li>Cut the screw threads into the acrylic piece that will be used to secure the ISCA. This can be achieved using a tap with a diameter and pitch matching the mounting screws.
		<ol>
			<li>First, affix the tap into a tap wrench, then secure the acrylic piece to be tapped in a benchtop vice. For the best results, make sure the acrylic piece is as level as possible. Make sure that the tap is perpendicular to the acrylic piece and start turning the tap wrench (clockwise), applying light pressure to the tap.</li>
			<li>After several full revolutions in the acrylic piece, reverse the rotation of the tap (counterclockwise) for one-quarter of a rotation to clear acrylic from the tap. Repeat the process until the entire depth of the acrylic piece is tapped.</li>
			<li>Finally, remove the tap (turning counterclockwise) and test the threads using a screw.</li>
		</ol>
		</li>
	</ol>
	</li>
</ol>
3. Procedure in the field
<ol>
	<li>Water filtration
	<ol>
		<li>Collect water from the field site when ready to start the experiment. Filter 5 mL of water per ISCA through a 0.2 &#181;m syringe filter (with a 50 mL syringe) into a 50 mL conical centrifuge tube. 
		NOTE: Approximately 3 mL of filtered water are required to fill all the wells of an ISCA; however, it is recommended to 1) filter 5 mL per device to account for losses during the quadruple filtration process, and 2) preserve aliquots of the filtrates as negative controls for both flow cytometry and molecular procedures.</li>
		<li>Filter the filtrate twice through a 0.2 &#181;m hydrophilic GP filter cartridge (using the same one, both times) with a new 50 mL syringe into a new 50 mL conical centrifuge tube. Filter the filtrate through a 0.02 &#181;m syringe filter (with a new 50 mL syringe) into a new 50 mL conical tube. 
		NOTE: This quadruple filtration should remove nearly all microorganisms and particles from the water. Keep the final filtrate away from any source of heat until use. This water will be used to resuspend all chemicals used in the ISCA, and it should be maintained at the same temperature as the water at the deployment site. Convective flows triggered by differences in temperature between the ISCA wells and outside environment may otherwise occur.</li>
		<li>Use aliquots of the filtrate to resuspend all chemoattractants of interest (typically dry) to the desired concentrations in 15 mL conical centrifuge tubes.</li>
		<li>Filter the resuspended chemoattractants through a 0.2 &#181;m syringe filter with a 10 mL syringe into sterile 15 mL conical centrifuge tubes to remove unwanted particles or water-insoluble compounds (if using extracts). 
		NOTE: Filter gently to prevent particles from passing through the filter. It is important to resuspend the chemoattractants in the ultrafiltered water from the field site and not solubilize them into other solutions. Using water from the field site is necessary to (1) obtain the same salt concentration inside the wells as that in the bulk environmental water to prevent density-driven flow, and (2) guarantee that background nutrient levels are equal inside and outside of the well.</li>
	</ol>
	</li>
	<li>ISCA filling
	<ol>
		<li>Perform section 1.3 to fill the ISCA. 
		NOTE: It is recommended to fill one row (five wells) per substance (i.e., three different substances per ISCA and one ultrafiltered seawater control).</li>
	</ol>
	</li>
	<li>Deployment in the field
	<ol>
		<li>Screw the ISCA (Figure 3A) to piece 9 of the enclosure (Figure 2K and Figure 3B). 
		NOTE: The flow-damping enclosure outlined above can contain two ISCAs side-by-side or one ISCA placed at its center.</li>
		<li>Close the enclosure (Figure 3C) and seal it with adhesive tape (Figure 3D). 
		NOTE: Wrinkles must be avoided to ensure a perfect seal. Seal all sides first, then (in a second step) seal the side holes, which will be used to drain water from the enclosure at the end of sampling. Do not seal the top and bottom holes. Do not place the ISCA upside down, as density-driven flow can occur in wells containing chemoattractants, which will bias the number of cells in the wells.</li>
		<li>Because the enclosure must remain steady during deployment, it is recommended to attach it to manmade structures (e.g., pontoon, ladder) using bungee cords. 
		NOTE: The enclosure can be attached to a deployment arm (here, a modified clamp with a perpendicular platform) using bungee cords before immersion in the water. Alternatively, the enclosure can be filled and secured with a small weight on shallow substrates. If deployments are intended in the pelagic ocean, the enclosure can be attached to a net with a buoy on one side and dive weight on the other.</li>
		<li>Submerge the enclosure completely to start filling. While filling, hold the enclosure firmly to prevent excessive water movement inside. Once the level of the water reaches the top of the enclosure, make sure that no air is trapped inside. 
		NOTE: In case some air bubbles are trapped, tilt the enclosure gently with the vent hole facing upward, which will enable the bubbles to escape.</li>
		<li>Once completely full, seal the bottom and top holes with two plugs, which can be made out of silicon or rubber or by sealing the extremities of 20 &#181;L pipette tips (Figure 4). 
		NOTE: This step prevents flow inside the enclosure during sampling.</li>
		<li>Leave the ISCA in place for sampling for 1&#8211;3 h. 
		NOTE: The ideal deployment time is primarily dictated by the temperature of the water and doubling time of the bacterial community. When the water temperature is above 20 &#176;C, it is not recommended to deploy the ISCA for more than 1 h, because cell division can occur in the wells containing chemoattractants after 1.5&#8211;2.0 h. However, optimal deployment time can be tested prior to the ISCA experiment by amending natural communities with the loaded chemicals and quantifying the number of cells through time.</li>
		<li>Remove the enclosure from the water. Place it over a container enabling the water to be drained from the enclosure.</li>
		<li>Remove the upper part of the adhesive tape from the front holes very gently. 
		NOTE: The flow of the water leaving the enclosure must be at a dripping speed. Proceed one hole at a time, from the top of the enclosure to the bottom. It should take approximately 10&#8211;15 min to drain the enclosure completely.</li>
		<li>Once the waterline passes below the top of the ISCA, remove the bottom plug, and drain the rest of the water.</li>
		<li>While the ISCAs are still attached to the enclosure, carefully remove the water trapped on top of the ISCA with a 1 mL pipette.</li>
		<li>Remove the ISCA without touching the upper surface and use a disposable wipe to remove any remaining liquid on the surface. 
		NOTE: It is important not to touch the ports during this process, as the resulting changes in pressure can remove or add bulk bacteria into the well and bias the bacterial counts.</li>
		<li>Retrieve the samples from the ISCA by repeating step 1.5.1.</li>
	</ol>
	</li>
</ol>
4. Downstream applications
NOTE: Volumes are given based on a 550 &#181;L sample (one row of an ISCA).
<ol>
	<li>Fix 100 &#181;L of well contents with glutaraldehyde (2% final concentration) for flow cytometry to quantify chemotaxis to each attractant. 
	NOTE: Store on ice (or at 4 &#176;C) and analyze the samples on the same day. Alternatively, samples can be flash frozen in liquid nitrogen following fixation if analysis is not feasible on the same day. Flow cytometry is the recommended method to quantify the number of cells in the ISCA wells, as it is straightforward, fast, and accurate12.</li>
	<li>Snap freeze 300 &#181;L of well content in liquid nitrogen for subsequent DNA extraction and analysis11. 
	NOTE: Store the samples at -80 &#176;C until analysis.</li>
	<li>Add 90 &#181;L of well contents to 10 &#181;L of TE-glycerol buffer and snap-freeze the samples for single-cell genomics13.</li>
	<li>Spread 10&#8211;20 &#181;L on agar plates containing the desired medium for bacterial isolation.</li>
</ol>

<ol><li>Stocker, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Marine+microbes+see+a+sea+of+gradients%5BTitle%5D)+AND+%22Science%22%5BJournal%5D)">Marine microbes see a sea of gradients.</a> Science. 338, 628-633 (2012).</li>
<li>Raina, J. B., Fernandez, V., Lambert, B., Stocker, R., Seymour, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+role+of+microbial+motility+and+chemotaxis+in+symbiosis%5BTitle%5D)+AND+%22Nature+Reviews+Microbiology%22%5BJournal%5D)">The role of microbial motility and chemotaxis in symbiosis.</a> Nature Reviews Microbiology. 17, 284-294 (2019).</li>
<li>Chet, I., Asketh, P., Mitchell, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Repulsion+of+bacteria+from+marine+surfaces%5BTitle%5D)+AND+%22Applied+Microbiology%22%5BJournal%5D)">Repulsion of bacteria from marine surfaces.</a> Applied Microbiology. 30, 1043-1045 (1975).</li>
<li>Smriga, S., Fernandez, V. I., Mitchell, J. G., Stocker, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chemotaxis+toward+phytoplankton+drives+organic+matter+partitioning+among+marine+bacteria%5BTitle%5D)+AND+%22PNAS%22%5BJournal%5D)">Chemotaxis toward phytoplankton drives organic matter partitioning among marine bacteria.</a> PNAS. 113, 1576-1581 (2016).</li>
<li>Matilla, M., Krell, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+effect+of+bacterial+chemotaxis+on+host+infection+and+pathogenicity%5BTitle%5D)+AND+%22FEMS+Microbiology+Reviews%22%5BJournal%5D)">The effect of bacterial chemotaxis on host infection and pathogenicity.</a> FEMS Microbiology Reviews. 42, (2018).</li>
<li>Adler, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chemotaxis+in+bacteria%5BTitle%5D)+AND+%22Science%22%5BJournal%5D)">Chemotaxis in bacteria.</a> Science. 153, 708-716 (1966).</li>
<li>Adler, J., Dahl, M. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+method+for+measuring+the+motility+of+bacteria+and+for+comparing+random+and+non-random+motility%5BTitle%5D)+AND+%22Journal+of+General+Microbiology%22%5BJournal%5D)">A method for measuring the motility of bacteria and for comparing random and non-random motility.</a> Journal of General Microbiology. 46, 161-173 (1967).</li>
<li>Ahmed, T., Shimizu, T. S., Stocker, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microfluidics+for+bacterial+chemotaxis%5BTitle%5D)+AND+%22Integrative+Biology%22%5BJournal%5D)">Microfluidics for bacterial chemotaxis.</a> Integrative Biology. 2, 604-629 (2010).</li>
<li>Hol, F. J. H., Dekker, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Zooming+in+to+see+the+bigger+picture%3A+microfluidic+and+nanofabrication+tools+to+study+bacteria%5BTitle%5D)+AND+%22Science%22%5BJournal%5D)">Zooming in to see the bigger picture: microfluidic and nanofabrication tools to study bacteria.</a> Science. 346, 1251821(2014).</li>
<li>Rusconi, R., Garren, M., Stocker, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microfluidics+expanding+the+frontiers+of+microbial+ecology%5BTitle%5D)+AND+%22Annual+Review+of+Biophysics%22%5BJournal%5D)">Microfluidics expanding the frontiers of microbial ecology.</a> Annual Review of Biophysics. 43, 65-91 (2014).</li>
<li>Lambert, B. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+microfluidics-based+in+situ+chemotaxis+assay+to+study+the+behaviour+of+aquatic+microbial+communities%5BTitle%5D)+AND+%22Nature+Microbiology%22%5BJournal%5D)">A microfluidics-based in situ chemotaxis assay to study the behaviour of aquatic microbial communities.</a> Nature Microbiology. 2, 1344-1349 (2017).</li>
<li>Marie, D., Partensky, F., Jacquet, S., Vaulot, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Enumeration+and+cell+cycle+analysis+of+natural+populations+of+marine+picoplankton+by+flow+cytometry+using+the+nucleic+acid+stain+SYBR+Green+I%5BTitle%5D)+AND+%22Applied+Environmental+Microbiology%22%5BJournal%5D)">Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow cytometry using the nucleic acid stain SYBR Green I.</a> Applied Environmental Microbiology. 63, 186-193 (1997).</li>
<li>Rinke, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Obtaining+genomes+from+uncultivated+environmental+microorganisms+using+FACS-based+single-cell+genomics%5BTitle%5D)+AND+%22Nature+Protocols%22%5BJournal%5D)">Obtaining genomes from uncultivated environmental microorganisms using FACS-based single-cell genomics.</a> Nature Protocols. 9, 1038-1048 (2014).</li>
<li>Gaworzewska, E. T., Carlile, M. J. Positive chemotaxis of Rhizobium leguminosarum and other bacteria towards root exudates from legumes and other plants. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aGaworzewska%2C+ET+%22Microbiology%22">Microbiology</a>. , (1982).</li>
<li>Walker, T. S., Bais, H. P., Grotewold, E., Vivanco, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Root+exudation+and+rhizosphere+biology%5BTitle%5D)+AND+%22Plant+Physiology%22%5BJournal%5D)">Root exudation and rhizosphere biology.</a> Plant Physiology. 132, 44-51 (2003).</li>
</ol>

The authors declare no conflict of interest.

At the scale of aquatic microorganisms, the environment is far from homogenous and is often characterized by physical/chemical gradients that structure microbial communities1,15. The capacity of motile microorganisms to use behavior (i.e., chemotaxis) facilitates foraging within these heterogeneous microenvironments1. Studying chemotaxis directly in the environment has the potential to identify important interspecific interactions and chem...

Diverse microorganisms use motility and chemotaxis to exploit patchy nutrient environments, find hosts, or avoid deleterious conditions1,2,3. These microbial behaviours can in turn influence rates of chemical transformation4 and promote symbiotic partnerships across terrestrial, freshwater, and marine ecosystems2,5.
Chemotaxis has been extensively studied under laboratory conditions for the past 60 years6. The first quantitative method to study chemotaxis, the capillary assay, involves a capillary tube filled with a putative chemoattractant immersed in a suspension of bacteria6. Diffusion of the chemical out of the tube creates a chemical gradient, and chemotactic bacteria respond to this gradient by migrating into the tube7. Since the development of the capillary assay, still widely used today, many other techniques have been developed to study chemotaxis under increasingly controlled physical/chemical conditions, with the most recent involving the use of microfluidics8,9,10.
Microfluidics, together with high-speed video microscopy, enables tracking of the behavior of single cells in response to carefully controlled gradients. Although these techniques have vastly improved our understanding of chemotaxis, they have been restricted to laboratory use and do not translate easily to field deployment in environmental systems. As a consequence, the capacity of natural communities of bacteria to use chemotaxis within natural ecosystems has not been examined; thus, current understanding of the potential ecological importance of chemotaxis is biased toward artificial laboratory conditions and a limited number of laboratory-cultured bacterial isolates. The recently developed ISCA overcomes these limitations11.
The ISCA builds on the general principle of the capillary assay; however, it makes use of modern microfabrication techniques to deliver a highly replicated, easily deployable experimental platform for the quantification of chemotaxis toward compounds of interest in the natural environment. It also allows identification and characterization of chemotactic microorganisms by direct isolation or molecular techniques. While the first working device was self-fabricated and constructed of glass and PDMS11, the latest injection-molded version is composed of polycarbonate, using a highly standardized fabrication procedure (for interest in the latest version of the device, the corresponding authors can be contacted).
The ISCA is credit card-sized and consists of 20 wells distributed in a 5 x 4 well array, each linked to the external aquatic environment by a small port (800 &#181;m in diameter; Figure 1). Putative chemoattractants loaded into the wells diffuse into the environment via the port, and chemotactic microbes respond by swimming through the port into the well. As many factors can influence the outcome of an ISCA experiment in the natural environment, this step-by-step protocol will help new users overcome potential hurdles and facilitate effective deployments.

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in situ chemotaxis assay to examine microbial behavior in aquatic ecosystems

In Situ Chemotaxis Assay to Examine Microbial Behavior in Aquatic Ecosystems

Microbial behaviors, such as motility and chemotaxis (the ability of a cell to alter its movement in response to a chemical gradient), are widespread across the bacterial and archaeal domains. Chemotaxis can result in substantial resource acquisition advantages in heterogeneous environments. It also plays a crucial role in symbiotic interactions, disease, and global processes, such as biogeochemical cycling. However, current techniques restrict chemotaxis research to the laboratory and are not easily applicable in the field. Presented here is a step-by-step protocol for the deployment of the in situ chemotaxis assay (ISCA), a device that enables robust interrogation of microbial chemotaxis directly in the natural environment. The ISCA is a microfluidic device consisting of a 20 well array, in which chemicals of interest can be loaded. Once deployed in aqueous environments, chemicals diffuse out of the wells, creating concentration gradients that microbes sense and respond to by swimming into the wells via chemotaxis. The well contents can then be sampled and used to (1) quantify strength of the chemotactic responses to specific compounds through flow cytometry, (2) isolate and culture responsive microorganisms, and (3) characterize the identity and genomic potential of the responding populations through molecular techniques. The ISCA is a flexible platform that can be deployed in any system with an aqueous phase, including marine, freshwater, and soil environments.

This section presents laboratory results using the ISCA to test the chemotactic response of marine microbes to a concentration range of glutamine, an amino acid known to attract soil bacteria14. The concentration of glutamine that elicited the strongest chemotactic response in the laboratory tests was used to perform a chemotaxis assay in the marine environment.
To perform the laboratory tests, seawater communities sampled from coastal water in Sydney, Australia, were e...

Presented here is the protocol for an in situ chemotaxis assay, a recently developed microfluidic device that enables studies of microbial behavior directly in the environment.

Microbial behaviors, such as motility and chemotaxis (the ability of a cell to alter its movement in response to a chemical gradient), are widespread ...

in-situ-chemotaxis-assay-to-examine-microbial-behavior-aquatic

Research

JoVE Journal

Environment

The fabrication of microfluidic channels and their implementation in experiments for studying the chemotactic foraging behaviour of marine microbes within a patchy nutrient seascape and the swimming behaviour of bacteria within shear flow are described.

Chemotactic Response of Marine Micro-Organisms to Micro-Scale Nutrient Layers

Studies of Bacterial Chemotaxis Using Microfluidics - Interview

This protocol describes the development of a microfluidic device for investigating bacterial chemotaxis in stable concentration gradients of chemoeffectors.

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

The ECIS/Taxis system is an automated, real-time assay that measures cellular chemotaxis.  In this assay, cells move beneath a layer of agarose to arrive at a target electrode.  Cellular movement is measured by the onset of resistance to AC current 0.

Measurement of Cellular Chemotaxis with ECIS/Taxis

A method of quantitatively evaluating the chemotactic response of Caenorhabditis elegans is described.  A chemotactic index (CI) was employed as a way to precisely evaluate the response of worms to certain targets, and serve as a platform of comparison between strains and compounds of interest.

C. elegans Chemotaxis Assay

This work describes a new protocol to assess the ecotoxicity of pollutants, including emerging contaminants such as nanomaterials, using the marine bacterium Vibrio anguillarum. This method allows for the determination of the LC50, or mortality, the concentration that causes a 50% decrease in bacteria culturability, after a 6 h exposure.

Ecotoxicological Method with Marine Bacteria Vibrio anguillarum to Evaluate the Acute Toxicity of Environmental Contaminants

We present a behavior recording setup and protocol that enables automated analysis of the nematode, Caenorhabditis elegans' preference for soluble compounds in a population-based assay. This article describes the construction of a behavior chamber, the behavioral assay protocol, and video analysis software usage.

Automated Analysis of a Nematode Population-based Chemosensory Preference Assay

A protocol for the generation of dynamic chemical landscapes by photolysis within microfluidic and millifluidic setups is presented. This methodology is suitable to study diverse biological processes, including the motile behavior, nutrient uptake, or adaptation to chemicals of microorganisms, both at the single cell and population level.

Generating Controlled, Dynamic Chemical Landscapes to Study Microbial Behavior

Here, we present an improved chemotaxis assay protocol. The goal of this protocol is to reduce the steps and costs of traditional bacterial chemotaxis methods and to serve as a valuable resource for understanding plant-microbe interactions.

An Improved Chemotaxis Assay for the Rapid Identification of Rhizobacterial Chemoattractants in Root Exudates

Flue gas from power plants is a cheap CO2 source for algal growth. We have built prototype "flue gas to algal cultivation" systems and described how to scale up the algal cultivation process. We have demonstrated the use of a mass-transfer bio-reaction model to simulate and to design the optimal operation of flue gas for the growth of Chlorella sp. in algal photo-bioreactors.

Optimize Flue Gas Settings to Promote Microalgae Growth in Photobioreactors via Computer Simulations

Learn in situ chemotaxis assay methods to study microbial behavior in aquatic ecosystems using the ISCA microfluidic device. This protocol enables robust quantification of chemotaxis and isolation of microorganisms.

In Situ Chemotaxis Assay to Examine Microbial Behavior in Aquatic Ecosystems

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