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Aardappelvirus X-Based microRNA Silencing (VbMS) In Aardappel.
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Aardappelvirus X-Based microRNA Silencing (VbMS) In Aardappel.

Article DOI: 10.3791/61067-v • 11:51 min • May 11th, 2020
May 11th, 2020 •
1, 1, 1,2
1Texas A&M AgriLife Research Center at Dallas, Texas A&M University System, 2Department of Plant Pathology, Microbiology, Texas A&M University

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We presenteren een gedetailleerd protocol voor het op aardappelvirus X (PVX) gebaseerde microRNA-uitschakelingssysteem (VbMS) om endogene microRNA's (miRNA's) in aardappelen functioneel te karakteriseren. Target mimic (TM) moleculen van miRNA van belang worden geïntegreerd in de PVX-vector en tijdelijk uitgedrukt in aardappel om de doel miRNA- of miRNA-familie tot zwijgen te brengen.

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Environmental Sciences virus-based microRNA silencing (VbMS) potato virus X (PVX) microRNA (miRNA) target mimic (TM) short tandem target mimics (STTMs) Solanum tuberosum
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