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JoVE Journal
Cancer Research

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Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres
 

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Article doi: 10.3791/61077
July 22nd, 2020

Summary July 22nd, 2020

Presented here is a protocol to discover the overexpressed driver genes maintaining the established cancer stem-like cells derived from colorectal HT29 cells. RNAseq with available bioinformatics was performed to investigate and screen gene expression networks for elucidating a potential mechanism involved in the survival of targeted tumor cells.

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