Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / Discovery of Driver Genes in Colorectal…
JoVE Journal
Cancer Research

A subscription to JoVE is required to view this content.

JoVE Journal Cancer Research
Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres
Non-Invasive Ultrasound Assessment of Endometrial Cancer Progression in Pax8-Directed Deletion of the Tumor Suppressors Arid1a and Pten in Mice
Journal (Cancer Research)
Non-Invasive Ultrasound Assessment of Endometrial Cancer Progression in Pax8-Directed Deletion of the Tumor Suppressors Arid1a and Pten in Mice
In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System
Journal (Cancer Research)
In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System
 

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Article DOI: 10.3791/61077-v • 06:52 min • July 22nd, 2020
July 22nd, 2020 •
1, 4, 1, 1,2,3
1Radiation Biology Research Center, Institute for Radiological Research, Chang Gung University, Chang Gung Memorial Hospital at Linkou, 2Department of Medical Imaging and Radiological Sciences, Chang Gung University, 3Department of Radiation Oncology, Chang Gung Memorial Hospital, 4Department of Laboratory Medicine, Mackay Memorial Hospital

Chapters

Summary
Automatic Translation

Presented here is a protocol to discover the overexpressed driver genes maintaining the established cancer stem-like cells derived from colorectal HT29 cells. RNAseq with available bioinformatics was performed to investigate and screen gene expression networks for elucidating a potential mechanism involved in the survival of targeted tumor cells.

Tags

Driver Genes Colorectal Cancer Tumorspheres RNA-Seq Bioinformatical Tools Overexpressed Genes Molecular Mechanism Cancer Stemness Inverted Microscope Digital Cell Imaging System Diameter Measurement Trypsin Treatment Cell Count Hemocytometer Centrifugation Antibody Incubation Flow Cytometry Analysis RNA Isolation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter