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Biology

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Reconstruction of Single-Cell Innate Fluorescence Signatures by Confocal Microscopy
 

Reconstruction of Single-Cell Innate Fluorescence Signatures by Confocal Microscopy

Article DOI: 10.3791/61120-v
May 27th, 2020

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Summary May 27th, 2020

Here, a protocol is presented for optically extracting and cataloging innate cellular fluorescence signatures (i.e., cellular autofluorescence) from every individual live cell distributed in a three-dimensional space. This method is suitable for studying the innate fluorescence signature of diverse biological systems at a single-cell resolution, including cells from bacteria, fungi, yeasts, plants, and animals.

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