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Reconstruction of Single-Cell Innate Fluorescence Signatures by Confocal Microscopy
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JoVE Journal Biology
Reconstruction of Single-Cell Innate Fluorescence Signatures by Confocal Microscopy

Reconstruction of Single-Cell Innate Fluorescence Signatures by Confocal Microscopy

DOI:
10.3791/61120-v

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07:29 min

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May 27, 2020

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, , , ,
1Graduate School of Life and Environmental Sciences,University of Tsukuba, 2Faculty of Life and Environmental Sciences,University of Tsukuba, 3Microbiology Research Center for Sustainability,University of Tsukuba

Chapters

  • 00:05Introduction
  • 01:00Microscope Setup
  • 01:50Image Acquisition
  • 02:502D Image Analysis
  • 04:22Statistical Analysis
  • 05:03Results: Representative Interspecies Segmentation and Variability Analyses
  • 06:46Conclusion

Summary

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Here, a protocol is presented for optically extracting and cataloging innate cellular fluorescence signatures (i.e., cellular autofluorescence) from every individual live cell distributed in a three-dimensional space. This method is suitable for studying the innate fluorescence signature of diverse biological systems at a single-cell resolution, including cells from bacteria, fungi, yeasts, plants, and animals.

Tags

Keywords: Single-cell AnalysisInnate FluorescenceConfocal MicroscopyMultichannel Confocal MicrospectroscopyCell IdentificationPhysiological CharacteristicsSpatial ResolutionPathogenic MicrobesPhenotypic HeterogeneityCell SegmentationFluorescence ImagingConfocal Reflection Microscopy

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