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JoVE Journal Biochemistry
Klickkemibaserad fluorometrisk analys för apolipoprotein N-acyltransferas från enzymkarakterisering till screening med hög genomströmning
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Klickkemibaserad fluorometrisk analys för apolipoprotein N-acyltransferas från enzymkarakterisering till screening med hög genomströmning

Article DOI: 10.3791/61146-v • 07:37 min • May 13th, 2020
May 13th, 2020 •
1, 1, 1
1Institut Pasteur, Unité Biologie et génétique de la paroi bactérienne, Paris, France ; CNRS, UMR 2001 « Microbiologie intégrative et Moléculaire », Paris, France ; INSERM, Équipe Avenir, Paris, France

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Här presenteras en känslig fluorescensanalys för att övervaka apolipoprotein N-acyltransferasaktivitet med diacylglycerylpeptid och alkyn-fosfolipider som substrat med klickkemi.

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Biokemi utgåva 159 integralmembranapolipoprotein N-acyltransferas diacylglycerylpeptid alkyn-fosfolipid blandade miceller klickkemi fluorescens
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