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Real-Time Imaging of CCL5-Induced Migration of Periosteal Skeletal Stem Cells in Mice
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Developmental Biology
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JoVE Journal Developmental Biology
Real-Time Imaging of CCL5-Induced Migration of Periosteal Skeletal Stem Cells in Mice

Real-Time Imaging of CCL5-Induced Migration of Periosteal Skeletal Stem Cells in Mice

DOI:
10.3791/61162-v

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06:10 min

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September 16, 2020

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, , ,
1Department of Molecular & Human Genetics,Baylor College of Medicine, 2Center for Skeletal Biology,Baylor College of Medicine, 3Department of Pathology & Immunology,Baylor College of Medicine

Chapters

  • 00:04Introduction
  • 00:39Surgical Procedure
  • 02:11CC Chemokine Ligand 5 (CCL5) Treatment
  • 02:50lntravital Imaging Preparation
  • 03:41Time-Lapse lmaging
  • 04:38Results: Representative Real-Time In Vivo Mx1 Alpha-SMA Periosteal Skeletal Stem Cells (P-SSC) Migration Imaging
  • 05:35Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the detection of CCL5-mediated periosteal skeletal stem cell migration in real-time using live animal intravital microscopy.

Tags

Keywords: Real-time ImagingCCL5Periosteal Skeletal Stem CellsMigrationIn Vivo Cell TrackingMicrofractureChemoattractantIntravital Imaging

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