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January 29, 2021
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We describe a reliable method for the preparation of whole cell extracts from yeast or other cells using a cryogenic freezer mill that minimizes degradation and denaturation of proteins. The cell extracts are suitable for purification of functional protein complexes, proteomic analyses, co-immunoprecipitation studies and detection of labile protein modifications.
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Cite this Article
Phillips, E. O. N., Giovinazzi, S., Menz, S. L., Son, Y., Gunjan, A. Preparation of Cell Extracts by Cryogrinding in an Automated Freezer Mill. J. Vis. Exp. (167), e61164, doi:10.3791/61164 (2021).
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