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Preparation of Cell Extracts by Cryogrinding in an Automated Freezer Mill
 

Preparation of Cell Extracts by Cryogrinding in an Automated Freezer Mill

Article doi: 10.3791/61164
January 29th, 2021

Summary January 29th, 2021

We describe a reliable method for the preparation of whole cell extracts from yeast or other cells using a cryogenic freezer mill that minimizes degradation and denaturation of proteins. The cell extracts are suitable for purification of functional protein complexes, proteomic analyses, co-immunoprecipitation studies and detection of labile protein modifications.

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