Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer

Xiangdong Lv; Lacey E. Dobrolecki; Yao Ding; Jeffrey  M. Rosen; Michael  T. Lewis; Xi Chen

doi:10.3791/61173

Journal

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Cancer Research

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Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer

5,963 Views

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07:45 min

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May 18, 2020

DOI:

10.3791/61173-v

DOI:

10.3791/61173-v

•

07:45 min

May 18, 2020

•

5947 Views

Xiangdong Lv*^1,2,3, Lacey E. Dobrolecki*^1,2,3, Yao Ding^1,2,3, Jeffrey M. Rosen^1,2,3, Michael T. Lewis^1,2,3, Xi Chen^1,2,3

¹Department of Molecular and Cellular Biology,Baylor College of Medicine ²Lester and Sue Smith Breast Center,Baylor College of Medicine ³Dan L. Duncan Cancer Center,Baylor College of Medicine

Summary

Patient-derived xenograft (PDX) models and transplantable genetically engineered mouse models faithfully recapitulate human disease and are preferred models for basic and translational breast cancer research. Here, a method is described to orthotopically transplant breast tumor fragments into the mammary fat pad to study tumor biology and evaluate drug response.