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Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer
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Chapters
- 00:04Introduction
- 01:22Cryopreserved Mammary Tumor Fragment Preparation
- 02:23Fresh Mammary Tumor Sample Collection and Preparation
- 03:28Surgical Preparation
- 04:04Tumor Fragment Transplantation
- 06:26Results: Representative Transplant Tumor Characterization
- 06:54Conclusion
Patient-derived xenograft (PDX) models and transplantable genetically engineered mouse models faithfully recapitulate human disease and are preferred models for basic and translational breast cancer research. Here, a method is described to orthotopically transplant breast tumor fragments into the mammary fat pad to study tumor biology and evaluate drug response.
