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Chapters
- 00:04Introduction
- 00:4296-Well Plate Coating
- 02:49Induced Pluripotent Stem Cell (iPSC) Culture Maintenance
- 05:03Cell Confluence Characterization
- 06:53Cytoskeletal Microfilament Characterization
- 09:02Results: Representative Analyses of Cell Confluence on Differently Coated Vessels
- 10:57Conclusion
Målet med protokollen er at sammenligne forskellige ekstracellulære matrix (ECM) belægningsbetingelser for at vurdere, hvordan differentiel belægning påvirker væksthastigheden for inducerede pluripotente stamceller (iPSC'er). Vi sigter især mod at skabe betingelser for at opnå optimal vækst af iPSC-kulturer.
