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Improved Fecundity and Fertility Assay for Aedes aegypti using 24 Well Tissue Culture Plates (EAgaL Plates)
JoVE Journal
Biology
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JoVE Journal Biology
Improved Fecundity and Fertility Assay for Aedes aegypti using 24 Well Tissue Culture Plates (EAgaL Plates)
DOI:

06:27 min

May 04, 2021

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Chapters

  • 00:04Introduction
  • 00:38Plate Preparation and Mosquito Feeding
  • 02:01Ovipositioning and Egg Counting
  • 04:25Fertility Assessment
  • 05:22Results: FeT Gene Silencing
  • 05:57Conclusion

Summary

Automatic Translation

Described is a time and space-saving method to count eggs and determine hatch rates of individual mosquitoes using 24 well tissue culture plates, which can substantially increase the scale and speed of fecundity and fertility assays.

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