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A Drosophila Model to Study Wound-induced Polyploidization
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
A Drosophila Model to Study Wound-induced Polyploidization

A Drosophila Model to Study Wound-induced Polyploidization

DOI:
10.3791/61252-v

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07:27 min

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June 09, 2020

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, , , ,
1Biology Department,Boston College, 2Graduate School of Biomedical Sciences and Engineering and Kathryn W. Davis Center for Regenerative Biology and Medicine, MDI Biological Laboratory,University of Maine

Chapters

  • 00:04Introduction
  • 00:32Adult Fruit Fly Staging and Wounding
  • 01:49Fly Abdomen Dissection
  • 04:00Stained Tissue Mounting
  • 04:57Results: Representative Re-Epithelization and Endoreplication Detection
  • 06:47Conclusion

Summary

Automatic Translation

Automatic Translation

Wound-induced polyploidization is a conserved tissue repair strategy where cells grow in size instead of dividing to compensate for cell loss. Here is a detailed protocol on how to use the fruit fly as a model to measure ploidy and its genetic regulation in epithelial wound repair.

Tags

Keywords: DrosophilaWound-induced PolyploidizationEpithelial CellsPloidyAbdominal WoundingGrace’s SolutionDissectionMicroscopy

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