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MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T

Article DOI: 10.3791/61266-v • 10:22 min • January 16th, 2021
January 16th, 2021 •
1, 2,3,4, 5, 2,4, 3,4, 1, 1,6
1Solid-state NMR, Leiden Institute of Chemistry, Faculty of Science, Leiden University, 2Laboratory of Biophysics, Wageningen University & Research, 3Laboratory of BioNanoTechnology, Wageningen University & Research, 4MAGNEtic resonance Facility, Wageningen University & Research, 5C.J. Gorter Center for High Field MRI, Radiology department, Leiden University Medical Centre, Leiden University, 6Institute for Medical Physics and Biophysics, Leipzig University

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A protocol to study biological tissue at high spatial resolution using ultra-high field magnetic resonance microscopy (MRM) using microcoils is presented. Step-by-step instructions are provided for characterizing the microcoils. Finally, optimization of imaging is demonstrated on plant roots.

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MRM Microcoil Performance Calibration Usage Medicago Truncatula Roots 22 T Ultra High Field Magnetic Resonance Imaging MRI Physiological Exploration Metabolic Exploration Diffusion Properties High Magnetic Field Strength Microcoils Cellular Resolution Imaging Characterization Commercial Microcoils Home-built Microcoils Radio Frequency Coil Adjustment Plant Root Imaging Signal To Noise Ratio Basic Parameters 90 Degree Pulse Length Pulse Power Safe Operating Limits Coil Sensitivity Calculation
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