Journal
/
Environment
/
Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica
JoVE Journal
Environment
Author Produced
This content is Free Access.
JoVE Journal Environment
Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica

Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica

DOI:
10.3791/61279-v

•

11:55 min

•

June 16, 2020

•

, , , ,
1Okinawa Institute of Science and Technology Graduate University, 2Francis Crick Institute, 3UCL Genetics Institute, Department of Genetics, Evolution and Environment,University College London

Chapters

  • 00:00Introduction
  • 00:45Initiation of Algal Culture
  • 02:41Field Collection of Oikopleura dioica
  • 04:04Isolation and Identification of O. dioica
  • 05:40O. dioica Culture Protocol
  • 07:38Preparation of Daily Food
  • 08:59Results
  • 10:23Conclusion

Summary

Automatic Translation

Automatic Translation

Oikopleura dioica is a tunicate model organism in various fields of biology. We describe sampling methods, species identification, culturing setup, and culturing protocols for the animals and algal feed. We highlight key factors that helped strengthen the culture system and discuss the possible problems and resolutions.

Tags

Keywords: Oikopleura DioicaPelagic TunicateCulture SystemAlgal FoodChaetoceros CalcitransPlankton NetField SamplingMarine EcologyModel Organism

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/61279?article_type=v

Integrated Field Lysimetry and Porewater Sampling for Evaluation of Chemical Mobility in Soils and Established Vegetation
check_url/61279?article_type=v

Soil Sampling and Isolation of Entomopathogenic Nematodes (Steinernematidae, Heterorhabditidae)
check_url/61279?article_type=v

Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies
check_url/61279?article_type=v

Modification and Application of a Leaf Blower-vac for Field Sampling of Arthropods
check_url/61279?article_type=v

Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis
check_url/61279?article_type=v

Sampling Soils in a Heterogeneous Research Plot
check_url/61279?article_type=v

A New Portable In Vitro Exposure Cassette for Aerosol Sampling
check_url/61279?article_type=v

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
check_url/61279?article_type=v

Cultivation of the Marine Pelagic Tunicate Dolioletta gegenbauri (Uljanin 1884) for Experimental Studies
check_url/61279?article_type=v

Microalgae Cultivation and Biomass Quantification in a Bench-Scale Photobioreactor with Corrosive Flue Gases

Read Article