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JoVE Journal Cancer Research
Assays voor het valideren van Histon Acetyltransferase Inhibitors
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Assays voor het valideren van Histon Acetyltransferase Inhibitors

Article DOI: 10.3791/61289-v • 09:11 min • August 6th, 2020
August 6th, 2020 •
1, 1
1Department of Anatomy and Cell Biology, and UF Health Cancer Center, University of Florida College of Medicine

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Remmers van histonacetyltransferases (HAT's, ook bekend als lysine acetyltransferasen), zoals CBP/p300, zijn potentiële therapieën voor de behandeling van kanker. Er zijn echter rigoureuze methoden nodig om deze remmers te valideren. Drie in vitro methoden voor validatie omvatten HAT-tests met recombinant acetyltransferases, immunoblotting voor histonacetylatie in de celcultuur en ChIP-qPCR.

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Kankeronderzoek lysine acetyltransferases KAT's CBP/p300 histonacetyltransferaseremmers screeningmethoden histonacetylatie epigenetica kanker genregulatie
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