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Continuous Measurement of Biological Noise in Escherichia Coli Using Time-lapse Microscopy
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JoVE Journal Bioengineering
Continuous Measurement of Biological Noise in Escherichia Coli Using Time-lapse Microscopy

Continuous Measurement of Biological Noise in Escherichia Coli Using Time-lapse Microscopy

DOI:
10.3791/61290-v

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08:25 min

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April 27, 2021

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, , , ,
1Department of Biomedical Engineering,Technion – Israel Institute of Technology

Chapters

  • 00:04Introduction
  • 00:58Escherichia coli Preparation and Plasmid Construction
  • 02:45Microscope Plate Preparation
  • 05:08Microscopic Imaging
  • 06:20Results: Representative Continuous Time-Lapse Microscopy Escherichia coli Biological Noise Analysis
  • 07:43Conclusion

Summary

Automatic Translation

Automatic Translation

This work presents a microscopy method that allows live imaging of a single cell of Escherichia coli for analysis and quantification of the stochastic behavior of synthetic gene circuits.

Tags

Keywords: Biological NoiseEscherichia ColiTime-lapse MicroscopyGenetic CircuitsSignal VariabilitySignal-to-noise RatioGFP ReporterBacterial CellLB BrothM9 Culture PlatesLow Melting AgarCasamino AcidMinimal Medium

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