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A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes
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A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes

A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes

DOI:
10.3791/61341-v

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09:59 min

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September 12, 2020

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, , , , , ,
1Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Science,Hunan Normal University, 2State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory for Microbial Molecular Biology, College of Life Science,Hunan Normal University, 3Department of Infectious Diseases, the Second Xiangya Hospital,Central South University

Chapters

  • 00:00Introduction
  • 01:30Midgut Dissection and Cultivable Bacteria Isolation
  • 04:55Antibiotic Treatment and Bacterium Reintroduction
  • 09:14Representative Results

Summary

Automatic Translation

Automatic Translation

This article presents a protocol to investigate the effect of individual mosquito gut bacteria, including isolation and identification of mosquito midgut cultivable microbes, antibiotic depletion of mosquito gut bacteria, and reintroduce one specific bacteria species.

Tags

Keywords: Bacterial Oral Feeding AssayAntibiotic-treated MosquitoesMosquito MidgutGut Microbiota16S RRNA Gene SequencingAntibiotic TreatmentColony Forming Unit AssayMosquito PhysiologyMidgut DissectionBacterial IsolationSurface Sterilization

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