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A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes

Article DOI: 10.3791/61341-v • 09:59 min • September 12th, 2020
September 12th, 2020 •
1,2, 1,2, 1,2, 1,2, 1,2, 3, 1,2
1Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Science, Hunan Normal University, 2State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory for Microbial Molecular Biology, College of Life Science, Hunan Normal University, 3Department of Infectious Diseases, the Second Xiangya Hospital, Central South University

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This article presents a protocol to investigate the effect of individual mosquito gut bacteria, including isolation and identification of mosquito midgut cultivable microbes, antibiotic depletion of mosquito gut bacteria, and reintroduce one specific bacteria species.

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Bacterial Oral Feeding Assay Antibiotic-treated Mosquitoes Mosquito Midgut Gut Microbes Host Metabolism Reproduction Fitness Vector Competence Gut Microbiota Removal 16S RRNA Gene Sequence Oral Feeding Assay Host Physiology Evaluation Midgut Dissection Cultivatable Bacteria Isolation
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