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Journal / Biochemistry / Spectrophotometric Screening for Potential…
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Biochemistry
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JoVE Journal Biochemistry
Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
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Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases

Article DOI: 10.3791/61347-v • 14:57 min • October 10th, 2020
October 10th, 2020 •
1,2, *1,3, *1,3
1Research platform of Pediatric Onco-Hematology, Department of Paediatrics, Gynaecology and Obstetrics, Faculty of Medicine, University of Geneva, 2Section of Biology, Faculty of Science, University of Geneva, 3Onco-Hematology Unit, Department of Women, Children-Adolescents, University Hospitals of Geneva
* These authors contributed equally

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Summary
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Glutathione S-transferases (GSTs) are detoxification enzymes involved in the metabolism of numerous chemotherapeutic drugs. Overexpression of GSTs is correlated with cancer chemotherapy resistance. One way to counter this phenotype is to use inhibitors. This protocol describes a method using a spectrophotometric assay to screen for potential GST inhibitors.

Tags

Spectrophotometric Screening Potential Inhibitors Cytosolic Glutathione S-transferases GSTs Metabolic Enzymes Intracellular Electrophilic Compounds MAP Kinases Apoptosis Pathway Drug Resistance Enzymatic Inhibitors Protocol Enzyme-compound Interaction Inhibitory Concentration 50 IC50 Constant Of Inhibition Ki Mode Of Inhibition Enzymatic Activity Protein Concentration Dilute Protein Solution 96-well Plate
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