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Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
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Bioengineering
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JoVE Journal Bioengineering
Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

DOI:
10.3791/61351-v

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08:15 min

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June 26, 2020

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, ,
1Department of Mechanical Engineering,Carnegie Mellon University, 2Department of Biomedical Engineering,Carnegie Mellon University, 3Department of Electrical and Computer Engineering,Carnegie Mellon University

Chapters

  • 00:04Introduction
  • 00:42Self-assembly Protocol for Multiple Distinct PNA Oligomers
  • 02:48Total Internal Reflection Fluorescence (TIRF) Microscopy Imaging
  • 05:08Different Morphologies for PNA Nanofibers in Varying Concentrations of SDS
  • 06:01Results: TIRF and TEM Imaging of PNA Nanofibers
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

This article provides protocols for the design and self-assembly of nanostructures from gamma-modified peptide nucleic acid oligomers in organic solvent mixtures.

Tags

Gamma-modified Peptide Nucleic AcidsNanostructuresSelf-assemblyOrganic Solvent MixturesDMSODMF14-dioxaneThermal AnnealingNitrocelluloseHumidity Chamber

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