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JoVE Journal
Immunology and Infection

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Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment
 

Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment

Article DOI: 10.3791/61359-v
June 24th, 2020

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Summary June 24th, 2020

This protocol describes the use of flow cytometry to identify the changes in immune cell composition, cytokine profile, and chemokine profile in the pulmonary environment following transient middle cerebral artery occlusion, a murine model of ischemic stroke.

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