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In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells
DOI:

06:01 min

December 03, 2020

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Chapters

  • 00:04Introduction
  • 00:59Induced Pluripotent Stem Cell (iPSC) Culture
  • 02:00iPSC Differentiation
  • 03:04Neuromuscular Junction (NMJ) Muscle Contraction and Curare Treatment
  • 03:47Results: Representative NMJ Phenotypic, Morphological, and Functional Characterization
  • 05:20Conclusion

Summary

Automatic Translation

Here we provide a protocol to generate in vitro NMJs from human induced pluripotent stem cells (iPSCs). This method can induce NMJs with mature morphology and function in 1 month in a single well. The resulting NMJs could potentially be used to model related diseases, to study pathological mechanisms or to screen drug compounds for therapy.

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