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In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells

In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells

DOI:
10.3791/61396-v

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06:01 min

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December 03, 2020

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, , ,
1Department of Clinical Application, Center for iPS Cell Research and Application (CiRA),Kyoto University, 2Department of Pediatrics,Kyoto Prefectural University of Medicine, 3Graduate Institute of Clinical Medicine,Taipei Medical University

Chapters

  • 00:04Introduction
  • 00:59Induced Pluripotent Stem Cell (iPSC) Culture
  • 02:00iPSC Differentiation
  • 03:04Neuromuscular Junction (NMJ) Muscle Contraction and Curare Treatment
  • 03:47Results: Representative NMJ Phenotypic, Morphological, and Functional Characterization
  • 05:20Conclusion

Summary

Automatic Translation

Automatic Translation

Here we provide a protocol to generate in vitro NMJs from human induced pluripotent stem cells (iPSCs). This method can induce NMJs with mature morphology and function in 1 month in a single well. The resulting NMJs could potentially be used to model related diseases, to study pathological mechanisms or to screen drug compounds for therapy.

Tags

Human Induced Pluripotent Stem CellsNeuromuscular JunctionMotor NeuronsSkeletal MuscleSchwann CellsSpinal Muscular AtrophyCell DifferentiationCell CultureCell DetachmentExtracellular MatrixDoxycyclineDifferentiation MediumMyogenic Differentiation MediumNeuromuscular Junction Medium

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