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Journal / Biochemistry / Mätning av nukleotidbindning till intakta,…
JoVE Journal
Biochemistry

This content is Open Access.

JoVE Journal Biochemistry
Mätning av nukleotidbindning till intakta, funktionella membranproteiner i realtid
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Mätning av nukleotidbindning till intakta, funktionella membranproteiner i realtid

Article DOI: 10.3791/61401-v • 08:33 min • March 11th, 2021
March 11th, 2021 •
1, 1, 1,2
1Department of Physiology, Anatomy and Genetics, University of Oxford, 2Department of Chemistry and Neuroscience Program, Trinity College

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Detta protokoll presenterar en metod för att mäta adeninnukleotidbindning till receptorer i realtid i en cellulär miljö. Bindning mäts som Förster-resonansenergiöverföring (FRET) mellan trinitrofenylnukleotidderivat och protein märkt med en icke-kanonisk, fluorescerande aminosyra.

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Denna månad i JoVE nummer 169 ligandbindning membranprotein jonkanal nukleotid metod lappklämma fluorescens spektra receptor
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