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JoVE Journal Neuroscience
Utilisation de neurones hippocampiques cultivés primaires pour étudier l’assemblage des segments initiaux axonaux
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Utilisation de neurones hippocampiques cultivés primaires pour étudier l’assemblage des segments initiaux axonaux

Article DOI: 10.3791/61411-v • 06:53 min • February 12th, 2021
February 12th, 2021 •
1,2,3, 2,3
1School of Basic Medical Sciences, Xi'an Jiaotong University, 2Department of Cell Biology, Duke University, 3Department of Biochemistry, Duke University

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Ici, nous avons décrit un protocole pour étudier quantitativement l’assemblage et la structure des segments initiaux d’axone (AIS) des neurones hippocampiques qui manquent d’AIS pré-assemblé en raison de l’absence d’un ankyrin-G géant.

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Rétraction Numéro 168 Ankyrine géante-G Axone segment initial Neurones cultivés primaires Transfection Imagerie Quantification de l’intensité AIS
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