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Journal / Biochemistry / Forbedret UPLC-UV-metode til kvantificering af…
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Biochemistry

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JoVE Journal Biochemistry
Forbedret UPLC-UV-metode til kvantificering af C-vitamin i salatsorter (Lactuca sativa L.) og Crop Wild Slægtninge (Lactuca spp.)
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Forbedret UPLC-UV-metode til kvantificering af C-vitamin i salatsorter (Lactuca sativa L.) og Crop Wild Slægtninge (Lactuca spp.)

Article DOI: 10.3791/61440-v • 10:22 min • June 30th, 2020
June 30th, 2020 •
*1,4, *2,4, 3, 1,4
1Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 2Unidad de Producción y Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 3Dpto Química Analítica, Escuela Politécnica Superior (Universidad de Zaragoza), 4Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza)
* These authors contributed equally

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Vi præsenterer en hurtig og pålidelig metode til kvantificering af C-vitamin i Lactuca spp. ved hjælp af UPLC-UV, potentielt kan overføres til andre planter. De vigtigste trin er prøvepræparatet og C-vitaminekstraktion under stabile forhold, reduktion af dehydroascorbinsyre til ascorbinsyre og optimering af den kromatografiske procedure.

Tags

Biokemi Ascorbinsyre Crop Wild Slægtninge (CWR) Dehydroascorbinsyre Salat Lactuca sativa L. Flydende kromatografi
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