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Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)

DOI:
10.3791/61440-v

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10:22 min

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June 30, 2020

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, , ,
1Unidad de Hortofruticultura,Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 2Unidad de Producción y Sanidad Animal,Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 3Dpto Química Analítica,Escuela Politécnica Superior (Universidad de Zaragoza), 4Instituto Agroalimentario de Aragón – IA2 (CITA-Universidad de Zaragoza)

Chapters

  • 00:04Introduction
  • 01:03Plant Material Preparation
  • 01:57Ascorbic Acid (AA) Standard Preparation
  • 02:34AA and Dehydroascorbic Acid (DHAA) Extraction
  • 03:40DHAA Reduction for Total AA (TAA) Extraction
  • 04:31Ultra-High-Performance Liquid Chromatography-Ultraviolet Preparation
  • 06:46AA Determination
  • 07:21AA and DHAA Quantification
  • 08:18Results: Representative Vitamin C Quantification in Lactuca Species
  • 09:44Conclusion

Summary

Automatic Translation

Automatic Translation

We present a fast and reliable method to quantify vitamin C in Lactuca spp. using UPLC-UV, potentially transferable to other plants. The key steps are the sample preparation and vitamin C extraction under stable conditions, the reduction of dehydroascorbic acid to ascorbic acid and the optimization of the chromatographic procedure.

Tags

Keywords: Vitamin CLettuceLactuca SativaLactuca Spp.UPLC-UVQuantificationExtractionAscorbic AcidDehydroascorbic AcidFood TechnologyNutritionPlant Breeding

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