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Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy
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Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy

Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy

DOI:
10.3791/61458-v

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10:12 min

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September 21, 2020

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, ,
1INM-Leibniz Institute for New Materials, 2Department of Physics,Saarland University

Chapters

  • 00:04Introduction
  • 00:48Cell Seeding and HER2 Labeling
  • 02:02Cleaning and Transferring Graphene onto Salt Crystals
  • 04:45Graphene Coating
  • 06:24STEM
  • 07:44Results: STEM of Graphene Coated SKBR3 Cells on Silicon Nitride Window
  • 09:38Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a protocol for labeling membrane proteins in mammalian cells and coating the sample with graphene for liquid-phase scanning transmission electron microscopy. The stability of the samples against the damage caused by radiation can also studied with this protocol.

Tags

Keywords: GrapheneElectron MicroscopyMammalian CellsLiquid-phaseCell ImagingHER2Antibody MimeticPMMA GrapheneSodium PersulfateSodium Chloride

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