Journal
/
Biology
/
Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers

Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers

DOI:
10.3791/61461-v

•

06:46 min

•

June 24, 2020

•

, , , , ,
1Maria Sklodowska-Curie National Research Institute of Oncology

Chapters

  • 00:04Introduction
  • 00:33Confluent Monolayer Generation
  • 01:06Focal Adhesion Analysis
  • 03:13Manual Cell Shape Analysis
  • 04:04Automated Cell Shape Analysis
  • 04:54Results: Representative Focal Adhesion and Cell Shape Distribution Analyses
  • 06:18Conclusion

Summary

Automatic Translation

Automatic Translation

The article describes quantification of 1) the size and number of focal adhesions and 2) cell shape index and its distribution from confocal images of the confluent monolayers of MCF7 cells.

Tags

Cell-substrate AdhesionCell ShapeMCF7 Cell MonolayersQuantificationConfocal MicroscopyImageJFocal Adhesion AnalysisBackground SubtractionBinary ConversionParticle AnalysisImageJ Macro

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Dissection of Organizer and Animal Pole Explants from Xenopus laevis Embryos and Assembly of a Cell Adhesion Assay

Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth

In situ Quantification of Pancreatic Beta-cell Mass in Mice

Imaging Cell Shape Change in Living Drosophila Embryos

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads

Quantification of Interbacterial Competition using Single-Cell Fluorescence Imaging

Automating Aggregate Quantification in Caenorhabditis elegans

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Read Article