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Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers
JoVE Journal
Biology
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JoVE Journal Biology
Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers
DOI:

06:46 min

June 24, 2020

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Chapters

  • 00:04Introduction
  • 00:33Confluent Monolayer Generation
  • 01:06Focal Adhesion Analysis
  • 03:13Manual Cell Shape Analysis
  • 04:04Automated Cell Shape Analysis
  • 04:54Results: Representative Focal Adhesion and Cell Shape Distribution Analyses
  • 06:18Conclusion

Summary

Automatic Translation

The article describes quantification of 1) the size and number of focal adhesions and 2) cell shape index and its distribution from confocal images of the confluent monolayers of MCF7 cells.

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