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Chapters
- 00:04Introduction
- 00:50Preparation for Tissue Dissection
- 01:36Zebrafish Brain Dissection and Single Nuclei Isolation
- 03:59FACS Based Nuclei Enrichment
- 05:16Results: Single Nuclei Isolation and Enrichment
- 06:04Conclusion
Single nuclei isolation relies on dissociation and detergent-based permeabilization of the cell membrane, steps that need optimization and are prone to introducing technical artifacts. We demonstrate a detergent and enzyme-free protocol for rapid isolation of intact nuclei directly from whole tissue, yielding nuclei suitable for single-nucleus RNA-seq (snRNA-Seq) or ATAC-seq.
