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Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method
Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method
JoVE Journal
Biology
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JoVE Journal Biology
Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

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07:00 min

June 24, 2020

DOI:

07:00 min
June 24, 2020

24524 Views
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Summary

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Single nuclei isolation relies on dissociation and detergent-based permeabilization of the cell membrane, steps that need optimization and are prone to introducing technical artifacts. We demonstrate a detergent and enzyme-free protocol for rapid isolation of intact nuclei directly from whole tissue, yielding nuclei suitable for single-nucleus RNA-seq (snRNA-Seq) or ATAC-seq.

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