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Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification
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Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Article DOI: 10.3791/61478-v • 07:25 min • June 25th, 2020
June 25th, 2020 •
1, 1, 2, 3, 4, 1
1Department of Plant Pathology, Coastal Plain Experiment Station, University of Georgia, 2University of Georgia Cooperative Extension, 3Department of Plant Pathology, Tropical Research & Education Center, University of Florida, 4Department of Agricultural and Environmental Sciences, Otis L. Floyd Nursery Research Center, Tennessee State University

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We developed a method to detect Phytophthora capsici zoospores in water sources using a filter paper DNA extraction method coupled with a loop-mediated isothermal amplification (LAMP) assay that can be analyzed in the field or in the lab.

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Phytophthora Capsici Irrigation Water Detection Loop-mediated Isothermal Amplification Waterborne Fighter Pathogen Rapid Detection Method Large Volume On Site Detection Owen Hudson Sumyya Waliullah Pump And Filter Setup Filtering Flask Hand Pump Buchner Funnel Filter Paper Water Samples Debris Sediment Soil Suction Forceps
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