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Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium
DOI:

09:10 min

July 14, 2020

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Chapters

  • 00:04Introduction
  • 00:44Capillary Electrode Filling
  • 01:51Microelectrode Holder Preparation
  • 02:46Electrode Test
  • 04:08Mouse and Electrode Positioning
  • 06:15Direct-Coupled Electroretinogram (DC-ERG) Recording
  • 07:04Results: Representative DC-ERG Traces and Workflow Analyses
  • 08:25Conclusion

Summary

Automatic Translation

Here, we present a method for recording light-evoked electrical responses of the retinal pigment epithelium (RPE) in mice using a technique known as DC-ERGs first described by Marmorstein, Peachey, and colleagues in the early 2000s.

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