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Medicine
Medicine
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生体材料に対する細胞毒性と細胞応答へのアクセス
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Article
DOI:
10.3791/61512-v
•
09:46 min
•
July 8th, 2021
生体材料に対する細胞毒性と細胞応答へのアクセス
July 8th, 2021
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Anabela Baptista Paula*1,2,3,4, Mafalda Laranjo*2,3,4,5, Ana Sofia Coelho1,2,3,4, Ana Margarida Abrantes2,3,4,5, Ana Cristina Gonçalves2,3,4,6, Ana Bela Sarmento-Ribeiro2,3,4,6, Manuel Marques Ferreira2,3,4,7, Maria Filomena Botelho2,3,4,5, Carlos Miguel Marto1,2,3,4,8, Eunice Carrilho1,2,3,4
1Institute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 2Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO), University of Coimbra, Coimbra, Portugal, 3Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Coimbra, Portugal, 4Clinical Academic Center of Coimbra, CACC, Portugal, 5Institute of Biophysics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 6Laboratory of Oncobiology and Hematology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 7Institute of Endodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, 8Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
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この方法論は、フローサイトメトリー、RT-PCR、免疫細胞化学、およびその他の細胞および分子生物学的手法を含む生存率アッセイおよび表現型分析を使用して、可溶性抽出物の調製を通じて生体材料の細胞毒性を評価することを目的としています。