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Obtaining Primary Osteocytes Through Murine Calvarial Fractionation of GFP-Expressing Osteocytes
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Obtaining Primary Osteocytes Through Murine Calvarial Fractionation of GFP-Expressing Osteocytes

Obtaining Primary Osteocytes Through Murine Calvarial Fractionation of GFP-Expressing Osteocytes

DOI:
10.3791/61513-v

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07:22 min

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June 02, 2020

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, , , , , , , , , ,
1Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine,Tohoku University Graduate School of Dentistry

Chapters

  • 00:05Introduction
  • 00:44Newborn Mouse Calvaria Dissection
  • 01:38Newborn Mouse Calvaria Fractionation
  • 03:47Osteocyte Preparation for Fluorescence Activated Cell Sorting
  • 05:13Results: Representative Osteocyte Characterization
  • 06:44Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the dissection of neonatal dmp1-topaz mouse calvaria and isolation of osteocytes expressing the green fluorescent protein through cell digestion and fractionation, in addition to osteocyte preparation for fluorescence activated cell sorting (FACS).

Tags

Keywords: Primary OsteocytesCalvarial FractionationGFP-expressing OsteocytesOsteocyte IsolationCell CultureCollagenaseEDTACell FractionationCell Purification

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