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opnåelse af primære osteocytter gennem murin calvarial fraktionering af GFP-ekspressive osteocytter
Quantitative Screening of Nanoparticle-Treated Fabric: The Log Reduction Method (Video) | JoVE
Journal (Biology)
Quantitative Screening of Nanoparticle-Treated Fabric: The Log Reduction Method (Video) | JoVE
Sample Preparation for Mass Cytometry Analysis
Journal (Biology)
Sample Preparation for Mass Cytometry Analysis
 
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opnåelse af primære osteocytter gennem murin calvarial fraktionering af GFP-ekspressive osteocytter

Article DOI: 10.3791/61513-v • 07:22 min • June 2nd, 2020
June 2nd, 2020 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine, Tohoku University Graduate School of Dentistry

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Denne protokol beskriver dissektion af neonatal dmp1-topas musecalvaria og isolering af osteocytter, der udtrykker det grønne fluorescerende protein gennem cellefordøjelse og fraktionering, ud over osteocytpræparation til fluorescensaktiveret cellesortering (FACS).

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Tilbagetrækning udgave 160 Primær osteocyt Dmp1 SOST GFP fraktionering FACS
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