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Journal / Biology / Oppnå primære osteocytter gjennom murine…
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Oppnå primære osteocytter gjennom murine calvarial fraksjonering av GFP-uttrykkende osteocytter
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Oppnå primære osteocytter gjennom murine calvarial fraksjonering av GFP-uttrykkende osteocytter

Article DOI: 10.3791/61513-v • 07:22 min • June 2nd, 2020
June 2nd, 2020 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine, Tohoku University Graduate School of Dentistry

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Denne protokollen beskriver disseksjon av neonatal dmp1-topaz mus calvaria og isolering av osteocytter som uttrykker det grønne fluorescerende proteinet gjennom cellefordøyelse og fraksjonering, i tillegg til osteocytpreparasjon for fluorescensaktivert cellesortering (FACS).

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Retraksjon utgave 160 primær osteocytt Dmp1 SOST GFP fraksjonering FACS
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